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File
language
eng
Attribute
technical note
Author
Asano Satoshi
Description
Mast cells are highly heterogenic cells meaning that different genes are expressed in each different population of mast cells. So far, it has been very difficult to detect that which individual mast cell expresses which genes in situ. In this study, we amplified the mouse mast cell protease (mmcp)-4 mRNAs by in situ RT-PCR method and successfully visualized the signals of amplicons. It was found that the cells expressing mmcp-4 mRNA were connective tissue type mast cells but not mucosal type mast cells, in mouse tongue and intestine sections. This is the first report that the cells expressing the mast cell-specific gene were detected on histological specimens by in situ RT-PCR method. And it is suggested that this method will be a powerful tool for clarifying the relationships between pathological and physiological changes in diseases and many genes expressed in mast cells.
Subject
in situ RT-RCR
mast cells
mouse mast cell protease
Journal Title
Shimane journal of medical science
Volume
33
Issue
1
Start Page
31
End Page
38
ISSN
03865959
ISSN(Online)
24332410
Published Date
2016-09-30
NCID
AA00841586
Publisher
Shimane University Faculty of Medicine
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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