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Title Transcription
タンシキン ヘノ プラスミド DNA ドウニュウ ト ソノ ハツゲン ニツイテノ ヨビテキ ケンキュウ
Title Alternative
Preliminary Study on the Trasfer of Plasmid DNA to Basidiomycetes and its Expression
File
language
eng
Author
Furuno, Takeshi
Shimada, Kenta
Matsuda, Hideyuki
Uehara, Tohru
Description
To attempt the gene transfer to the basidiomycetes such as wood-decaying fungi, the experimental system of transferring plasmid DNA and seeing the expression was investigated. Using two species of Tyromyces palustris and Coriolus versicolor, their protoplasts from mycelia cultivated in SMY liquid culture media were prepared by treatments with the cell wall-lysing enzymes and others. As plasmid DNA, pUC4K having a kanamycin-resistant gene was selected because of obstructing the growth of mycelia. The transfer of plasmid DNA was examined by two ways of polyethylene glycol and electro poration.
The transformants of Tyromyces palustris obtained by electroporation showed obviously a faster growth than the non-transformed cells in kanamycincontaining liquid media. As a result of agarose gel electrophoresis for chromosomal DNA and plasmid DNA extracted from these transformants, the bands could be detected at the same position of plasmid pUC4K. This verified the transformation to Tyromyces palustris by transferring pUC4K.
Keywords: transformation; basidiomycetes; Tyromyces palustris; Plasmid pUC4K; electroporation.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
28
Start Page
83
End Page
92
ISSN
0370940X
Published Date
1994-12-20
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Shimane University
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