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Title Transcription
ショウサンエン カンゲンセイ サイキン ノ カンベン シキベツホウ
Title Alternative (English)
A Rapid Method for the Differentiation of Nitrate Reducer by Reagent-Agar Overlay Technique
File
language
jpn
Author
Matsumoto, Muneto
Nishitai, Yujiro
Kayashima, Takayuki
Description
A rapid plate method is described for the differentiation of nitrate reducer in lieu of the conventional liquid culture method. The method includes an ordinary plate culture with bouillon-agar, subsequent treatment with overlaying agar containing nitrate and Griss-Romin reagent on the plate culture, and counting of red colonies developed. With a considerable seving of time, trouble, and culture media, easy estimation of the population ratio of nitrate reducer in the natural environment was attained by this method. By modifying the pH value of the original Griss Romin reagent from 2.2 to 4.0, more viable cells were able to be picked up with less reagent injury from the plate after the differentiation test.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
13
Start Page
112
End Page
115
ISSN
0370940X
Published Date
1979-12-15
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science
Remark
開学30周年記念号
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