In order to investigate the conductive passive way of sap flow in the trunk of a Cryptomeria Japonica D.Don standing tree, the tree was cut down after the trunk was made to freeze directly by liquid nitrogen. In respect of the moisture profile of taken out freezing sample moistures satatus was visualized by utilizing the soft X-ray and the Cryo-SEM. And, in addition to them, the conductive passive way of sap flow was observed by the saftanine staining. Results obtained are summarized as follows :
1. The water existed in almost all the regions of sapwood in the trunk at the road clearance of about 1.2m. With approaching the white zone, the number of tracheid in which contains less water increased. In the white zone, the water hardly existed. Though the contents existed in almost all the traacheid of the heartwood, they were not only water but also heartwood materials. And more, in the region from the sapwood to the white zone, the water existed in almost all the latewood.
2. Almost the whole area of the sapwood at the position of 5mm higher than the position that injected the safranine(road clearance : 1.26m, H_<1.26>) was stained by safranine. And, in the white zone near the sapwood, it was recoginzed that only the latewood stained by safranine. In the sapwood at the position of 227 mm higher than H_<1.26>, the earlywood was mainly stained by safranine. And, in the white zone near the sapwood, only the latewood stained by safranine as well as the previous position. And more, at the highest stained position, though the annual ring of current year was not stained by safranine, only the earlywood near the latewood in the annual ring of last year was stained by safranine. Finally, the heartwood at all measured positions was not stained by safranine.