Shimane Journal of Medical Science

Shimane University Faculty of Medicine
ISSN :0386-5959(冊子体)
ISSN :2433-2410(オンライン)

クリエイティブ・コモンズ・ライセンス
これらの論文は クリエイティブ・コモンズ 表示 - 非営利 - 改変禁止 4.0 国際 ライセンスの下に提供されています。
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Shimane Journal of Medical Science 9 1
1985-11-01 発行

Effects of Chlorambucil on the Ultrastructure of Neuroepithelial Cells of Neurulating Mouse Embryos

篠原 春夫
吉岡 孝文
田中 修
ファイル
内容記述(抄録等)
The effects of chlorambucil on the neuroepithelial cells of mouse embryos are described ultrastructurally. Pregnant ICR mice were treated with oral administration of 3mg/kg chlorambucil at 7th day of gestation. Embryos were collected 6,12,24,36,and 48 hours after treatment and used for ultrastructural study. At 6 hours after treatment, many pyknotic cells were observed in the neural fold, which were thought to be the result of the direct cytotoxic effect of chlorambucil. At 24 hours, electron-dense protrusions of cytoplasm into the lumen of neural tube, which we call cytoplasmic clump, were observed. Intranuclear inclusions, which seemed to originate from invagination of the inner membrane of the nuclear envelope, were also observed. They were thought to be the characteristic findings in hypermetabolic cells induced by chlorambucil. At 36-48 hours pyknotic cells had almost disappeared, indicating that repair had taken place.