Shimane Journal of Medical Science

Shimane University Faculty of Medicine
ISSN :0386-5959(冊子体)
ISSN :2433-2410(オンライン)

クリエイティブ・コモンズ・ライセンス
これらの論文は クリエイティブ・コモンズ 表示 - 非営利 - 改変禁止 4.0 国際 ライセンスの下に提供されています。
ダウンロード数 : ?
Shimane Journal of Medical Science 33 1
2016-09-30 発行

Histological Detection of mmcp-4 Expressing Cells by In Situ RT-PCR Method

Asano Satoshi
新林 友美
ファイル
内容記述(抄録等)
Mast cells are highly heterogenic cells meaning that different genes are expressed in each different population of mast cells. So far, it has been very difficult to detect that which individual mast cell expresses which genes in situ. In this study, we amplified the mouse mast cell protease (mmcp)-4 mRNAs by in situ RT-PCR method and successfully visualized the signals of amplicons. It was found that the cells expressing mmcp-4 mRNA were connective tissue type mast cells but not mucosal type mast cells, in mouse tongue and intestine sections. This is the first report that the cells expressing the mast cell-specific gene were detected on histological specimens by in situ RT-PCR method. And it is suggested that this method will be a powerful tool for clarifying the relationships between pathological and physiological changes in diseases and many genes expressed in mast cells.