Shimane Journal of Medical Science

Shimane University Faculty of Medicine
ISSN :0386-5959(冊子体)
ISSN :2433-2410(オンライン)

クリエイティブ・コモンズ・ライセンス
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Shimane Journal of Medical Science 2 1
1978-09-01 発行

Immunohistochemical Localization of Lactate Dehydrogenase Subunits in Porcine Liver ; Effect of Fixatives on Detection of Specific Fluorescence

伊藤 元彦
Fukuda Haruo
鈴木 幸雄
原田 孝之
森川 茂
ファイル
内容記述(抄録等)
Immunohistochemical localization of lactate dehydrogenase subunits was studied using a fluorescent antibody technique in porcine liver. Because of the difficulty in demonstrating specific fluorescence of LDH-M in the liver tissue, many fixatives were tested both in frozen and paraffin sections to obtain consistent staining results. Among the fixatives examined, ether-ethanol-acetone mixture in frozen sections gave the best result in demonstrating both subunits in the liver tissue by immunofluorescence. Paraffin sections failed to preserve LDH-M specific fluorescence in this study. Specific fluorescence for LDH-H was observed in the hepatic cell membrane facing the space of Disse, in the lining cells of sinusoids and in the cyto plasm of Kupffer cells. LDH-M specific fluorescence was observed as fine granules in the cytoplasm of hepatic cells.