Shimane University Faculty of Medicine
ISSN :0386-5959(冊子体)
ISSN :2433-2410(オンライン)
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Shimane Journal of Medical Science 17 1
1999-06-01 発行
Detection of cathepsin B-like enzyme activity in mouse epidermis
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内容記述（抄録等）
In this study, we could detect cathepsin B-like, i.e., BANA-hydrolysing, enzymeactivity in the cytosolic fraction of mouse epidermal lysate. The optimum pH and the optimum temperature of this enzyme activity were pH 6.0 and 37℃, respectively. The BANA hydrolysis occured dose dependently to the protein amount of the cytosolic
fraction and, lupeptin, which is a specific inhibitor to cathepsin B, was strongly inhibited BANA hydrolysis at pH 6.0 under 37℃ condition.
fraction and, lupeptin, which is a specific inhibitor to cathepsin B, was strongly inhibited BANA hydrolysis at pH 6.0 under 37℃ condition.
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