Shimane Journal of Medical Science

Shimane University Faculty of Medicine
ISSN :0386-5959(冊子体)
ISSN :2433-2410(オンライン)

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Shimane Journal of Medical Science 11
1988 発行

In vitro ADP-Ribosylation of AMP-Deaminase and Its Effect on the Protection of the ADP-Binding Domain against Proteolysis

Haroutunian A., V.
三島 宏一
谷河 精規
下山 誠
ファイル
内容記述(抄録等)
We investigated the ADP-ribosylation of AMP-deaminase from rat and rabbit skeletal muscle by guanidino compound-specific ADP-ribosyltransferase from hen liver nuclei and its effect on the enzyme activity. Rat AMP-deaminase was ADP-ribosylated when the enzyme was incubated with ADP-ribosyl-transferase and [adenylate-^32P] NAD. Preincubation of rabbit AMP-deaminase alone resulted in a loss of ADP-dependent, but not basal, enzyme activity. If, however, the enzyme was subjected to the ADP-ribosylation system containing unlabeled NAD and ADP-ribosyltransferase, the enzyme retained the property to be activated by ADP. Electrophoretic analyses of the enzyme preparation incubated with or without the ADP-ribosylation system and subsequently further incubation with trypsin showed that the ADP-ribosylation of the enzyme protects the enzyme from proteolysis concomitant with the retention of ADP-dependent activation of the enzyme.