Dermatophytes were isolated from lesions of dermatophytosis in the Izumo City and its vicinity, Japan. From the 106 lesions, 107 strains were isolated. Among the isolates from each type of dermatophytosis as well as among the whole isolates, the most frequently isolated dermatophytes were Trichophyton rubrum or Trichophyton mentagrophytes. Epidermophyton floccosum was also a frequent dermatophyte both among the whole isolates and among the isolates from each type of dermatophytosis. This high isolation frequency of Epidermophyton floccosum is unusual.