島根大学教育学部紀要. 自然科学

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島根大学教育学部紀要. 自然科学 35
2001-12-01 発行

ヒト染色体を保有するキメラマウス胎仔における発生異常

Developmental anomalies in the foetuses of a chimeric mouse containing a human chromosome
舟木 賢治
ファイル
内容記述(抄録等)
Chimeric mice containing a human chromosome were produced by introducing a human chromosome into mouse embryonic stem cells via microcell-mediated chromosome transfer.
In present study, the dead foetuses of the chimeric mice containing a human chromosome 2-derived fragment (hChr.2f) or a human chromosome 21 (hChr.21) were studied anatomically in order to examine the effects of introduction of a human chromosome to mouse. Polydactyly were obsered in almost of the chimeric foetuses containing hChr.2f, but were not detected in the chimeric foetuses containing hChr.21. Aplasia of the sternum and delayed ossification or deletion of the cervical vertebrae centrum were universally observed in both the chimericfoetuses. Histological abnormalities in heart and liver were frequently detected in both the chimeric foetuses. Interestingly, the number of germ cells decreased remarkably in all of the chimeric foetuses containing hChr.21.