Fauna of Drosophilidae was studied at three locations,Matsue Castle Park, Rakuzan Park and Mt. Dake-san in 1993 and 2005 to 2006. Forty five species of thirteen genera of Drosophilidae were identified and Nesiodrosophila delicate and Dichaetophora surukella were the first collection record in Chugoku district. Drosophila simulans that spreads in Japan mainland from 1970s was collected more in 2005 to 2006 than in 1993 while D. albomicans that spreads from 1980s in mainland was collected only in 1993. Our collection data was compared to collection record by Wakahama at Rakuzan Park and Mt. Dake-san. Scaptodrosphila subtilis and D. bizonata were dominant species in 1957 to 1961 but only accounted for less than 5％ in our study. We collected a plenty number of D. immigrans more than15％ of collected flies, but it was not so abundant in 1957 to 1961. Sca. coracina was conspicuous in 2005 to 2006. The cosmopolitan domestic species was increased in its frequency at all sites.