Bulletin of the Faculty of Life and Environmental Science Shimane University

number of downloads : ?
Bulletin of the Faculty of Life and Environmental Science Shimane University 17
2012-09-30 発行

Bioavailability of rumen protozoan selenium fed to mice using tissue uptake technique

Fujihara, Tsutomu
File
Description
In the present study, the utilization of protozoan selenium(Se)synthesized in the rumen of sheep was investigated in mice using the rate of Se incorporation into the blood, organs and tissues as an index. Protozoan Se was prepared from rumen content of sheep supplemented Na selenite(0.4mg/kg DM of diet). Twenty female mice(over8-week-age)were divided two groups of10each, and ten of them fed the diet containing rumen protozoa matter(rpm:100g Se/kg DM)during consecutive7days, and another 10mice were dealt with as control group. Then, all the animals were slaughtered to collect whole blood, kidney, liver, pancreas, spleen, lung, heart, brain and muscles for analyzing Se. The Se contents in protozoa and bacteria in the rumen of sheep supplemented Na selenite(0.4mg/ Kg DM)was0.45mg /Kg DM and0.20mg/ Kg DM, respectively. In the concentration of Se in blood, organs and tissues, the values tended to higher in mice fed rpm diet than in control group. The Se contents in blood, organs and muscles also tended to high in the group fed rpm diet compared with that in control group. From these findings, it can be obviously concluded that the protozoan Se incorporated in the rumen is efficiently utilized in animals.