ダウンロード数 : ?
ファイル
言語
英語
著者
GOLDAR, Md Muniruzzaman Toshikazu NISHIE
NISHIE, Toshikazu Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
ISHIKURA, Yasue Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
FUKUDA, Toshifumi Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
TAKEGAWA, Kaoru Department of Life Sciences, Faculty of Agriculture, Kagawa University
内容記述(抄録等)
The moc1/sds23 gene was isolated to induce sexual development of a sterile strain due to overexpression of adenylate cyclase in Schizosaccharomyces pombe. Here, we studied the functional conservation between moc1/sds23 and its two orthologs SDS23 and SDS24 in Saccharomyces cerevisiae. We observed that the temperature sensitivity, salt tolerance, cell morphology, and sterility of the Δmoc1 mutant in S. pombe were recovered by expressing either S. cerevisiae SDS23 or SDS24. We found that deletion of both SDS23 and SDS24 resulted in the production of a large vacuole that was reversed by the expression of S. pombe moc1/sds23. In these ways we found that S. pombe Moc1/Sds23 and S. cerevisiae SDS23p or SDS24p are functional homologs. In addition we found that the Δsds23 Δsds24 diploid strain reduces cell separation in forming pseudohyphal-like growth in S. cerevisiae. Thus S. pombe moc1/sds23 and S. cerevisiae SDS23 or SDS24 are interchangeable with each other, but their disruptants are phenotypically dissimilar.
主題
Schizosaccharomyces pombe
Saccharomyces cerevisiae
moc1
vacuole
pseudohyphae
掲載誌名
Bioscience, Biotechnology, and Biochemistry
69
7
開始ページ
1422
終了ページ
1426
ISSN
0916-8451
ISSN(Online)
1347-6947
発行日
2005-01-01
DOI
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
著者版
部局
生物資源科学部