ダウンロード数 : ?
ファイル
言語
英語
著者
YAKURA, Miyo Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
ISHIKURA, Yasue Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
ADACHI, Yoshimi Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University
内容記述(抄録等)
The moc1 gene in Schizosaccharomyces pombe was found as to overcome sterility caused by high expression of adenylyl cyclase. The moc1 gene was found to be identical with sds23 and psp1. Although psp1 has been reported to be essential for growth, sds23 has not been. To clarify this apparent discrepancy, we first assessed independently the phenotypes of the moc1 disruptant. We confirmed that the deletion mutant of moc1 is sterile, sensitive to high salt, and grows slowly at higher and lower temperatures, and that mutant cells are elongated. Besides these phenotypes, we found that viability of the moc1 disruptant was rapidly lost at the stationary phase. We confirmed that the Moc1 protein is phosphorylated in the stationary phase and also under nitrogen-starved conditions. We examined the significance of this phosphorylation of Moc1 by creating the S333A or S333D mutant Moc1. Interestingly, while S333D mutant Moc1 is lower in inducing sexual development, S333A mutant Moc1 is higher in this than the wild type, suggesting that phosphorylation of Moc1 affects sexual development. The other phenotypes, such as sensitivity to high salt and higher temperature and elongation of cells, were not affected by mutation of S333A nor S333D. We found that Moc1-GFP localized to both the cytosol and the nucleus during mitotic growth, but accumulated in the nucleus in mating cells and then enriched in spores, and that this localization shift was negatively regulated by the cAMP pathway. This and the observations above suggest that nuclear localized Moc1 is an inducer of sexual development. Thus, in addition to the roles of moc1/sds23/psp1 in mitosis and stress response, it is also important for the survival and sexual development of fission yeast, but phosphorylation of Moc1 only affects the sexual development.
主題
Schizosaccharomyces pombe
sexual development
meiosis
moc1
掲載誌名
Bioscience, Biotechnology, and Biochemistry
70
7
開始ページ
1740
終了ページ
1749
ISSN
0916-8451
ISSN(Online)
1347-6947
発行日
2006-07-23
DOI
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
著者版
部局
生物資源科学部