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タイトルヨミ
ケッセイ アルブミン ノ PH コウバイ ブンパイ クロマトグラフィ
タイトル別表記
A pH Gradient Partition Chromatography of Plasma Albumin : An Attempt se Separate into Subfractions
ファイル
言語
英語
著者
久下 喬
内容記述(抄録等)
It has been pointed out that plasma albumin undergoes a transformation between N and F forms depending on pH at a pH region 3.0-5.0(2). Sogami and Foster(8) recently suggested that there might exist a large number of closely related albumin species having slightly different types of tertiary structures.
Each species has its own characteristic pH at which the N-F transformation takes place, and the transformation is extremely sharp being accompanied by a 12 hydrogen transfer. Assuming a Gaussian type distribution of a population of these species, we can properly explain the observed pH dependence of the N-F transformation.
Because of the much lower water solubility of F form and its enhanced solubility in organic solvents (5) , partition chromatography seems to be useful to separate albumin into two subfractions corresponding to N and F forms. Moreover, we can expect that many albumin species might be separated by a pH gradient elution because of their characteristic pH dependences.
Under these considerations, a pH gradient partition chromatography was attempted for plasma albumin.
掲載誌名
島根農科大学研究報告
13
開始ページ
(A-3)116-120
ISSN
05598311
発行日
1965-01-10
NCID
AN00108241
出版者
島根農科大学
出版者別表記
The Shimane Agricultural College
資料タイプ
紀要論文
部局
生物資源科学部
備考
A,Bを含む