ファイル
言語
英語
著者
伊藤 元彦
Fukuda Haruo
鈴木 幸雄
原田 孝之
森川 茂
内容記述(抄録等)
Immunohistochemical localization of lactate dehydrogenase subunits was studied using a fluorescent antibody technique in porcine liver. Because of the difficulty in demonstrating specific fluorescence of LDH-M in the liver tissue, many fixatives were tested both in frozen and paraffin sections to obtain consistent staining results. Among the fixatives examined, ether-ethanol-acetone mixture in frozen sections gave the best result in demonstrating both subunits in the liver tissue by immunofluorescence. Paraffin sections failed to preserve LDH-M specific fluorescence in this study. Specific fluorescence for LDH-H was observed in the hepatic cell membrane facing the space of Disse, in the lining cells of sinusoids and in the cyto plasm of Kupffer cells. LDH-M specific fluorescence was observed as fine granules in the cytoplasm of hepatic cells.
主題
LDH subunits
fluorescent antibody
liver
掲載誌名
Shimane journal of medical science
2
1
開始ページ
25
終了ページ
31
ISSN
03865959
ISSN(Online)
24332410
発行日
1978-09-01
NCID
AA00841586
出版者別表記
Shimane Medical University
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
部局
医学部