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ファイル
言語
英語
著者
ChoongSoo Yun Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan
Amakata, Daiki Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan
松田 英幸
内容記述(抄録等)
The betaproteobacterium Mitsuaria chitosanitabida (formerly Matsuebacter chitosanotabidus) 3001 produces a chitosanase (ChoA) that is classified in glycosyl hydrolase family 80. While many chitosanase genes have been isolated from various bacteria to date, they show limited homology to the M. chitosanitabida 3001 chitosanase gene (choA). To investigate the phylogenetic distribution of chitosanases analogous to ChoA in nature, we identified 67 chitosan-degrading strains by screening and investigated their physiological and biological characteristics. We then searched for similarities to ChoA by Western blotting and Southern hybridization and selected 11 strains whose chitosanases showed the most similarity to ChoA. PCR amplification and sequencing of the chitosanase genes from these strains revealed high deduced amino acid sequence similarities to ChoA ranging from 77% to 99%. Analysis of the 16S rRNA gene sequences of the 11 selected strains indicated that they are widely distributed in the β and γ subclasses of Proteobacteria and the Flavobacterium group. These observations suggest that the ChoA-like chitosanases that belong to family 80 occur widely in a broad variety of bacteria.
主題
Betaproteobacteria
Chitosan
Flavobacterium
Gammaproteobacteria
glycoside hydrolases
掲載誌名
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
71
9
開始ページ
5138
終了ページ
5144
ISSN
0099-2240
ISSN(Online)
1098-5336
発行日
2005-09
DOI
出版者
American Society for Microbiology
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
著者版
部局
生物資源科学部