ダウンロード数 : ?
ファイル
言語
英語
著者
Kaino, Tomohiro
Tonoko, Kai
Mochizuki, Shiomi
Takashima, Yuriko
内容記述(抄録等)
Coenzyme Q (CoQ) is essential for mitochondrial respiration and as a cofactor for sulfide 3 quinone reductase. Schizosaccharomyces pombe produces a human-type CoQ10. Here, we 4 analyzed CoQ in other fission yeast species. S. cryophilus and S. octosporus produce 5 CoQ9. S. japonicus produces low levels of CoQ10, although all necessary genes for CoQ 6 synthesis have been identified in its genome. We expressed three genes (dps1, dlp1, and 7 ppt1) for CoQ synthesis from S. japonicus in the corresponding S. pombe mutants, and 8 confirmed that they were functional. S. japonicus had very low levels of oxygen 9 consumption and was essentially respiration defective, probably due to mitochondrial 10 dysfunction. S. japonicus grows well on minimal medium during anaerobic culture, 11 indicating that it acquires sufficient energy by fermentation. S. japonicus produces 12 comparable levels of ethanol under both normal and elevated temperature (42°C) 13 conditions, at which S. pombe is not able to grow.
主題
Fission yeast
Schizosaccharomyces japonicus
S. pombe
respiration
CoQ
掲載誌名
Biosci Biotechnol Biochem
82
6
開始ページ
1031
終了ページ
1042
ISSN
0916-8451
ISSN(Online)
1347-6947
発行日
2018-6
DOI
PubMed ID
出版者
公益社団法人日本農芸化学会
資料タイプ
学術雑誌論文
著者版/出版社版
著者版
部局
生物資源科学部
備考
This is an electronic version of an article published in Bioscience, Biotechnology, and Biochemistry [Volume.82 No.6, pp.1031-1042, 2018 ]. Bioscience, Biotechnology, and Biochemistry is available online at: https://www.tandfonline.com/doi/full/10.1080/09168451.2017.1401914