ダウンロード数 : ?
ファイル
言語
英語
日本語以外のタイトル
Evaluation of the inhibitory effect of docosahexaenoic acid and arachidonic acid on the initial stage of amyloid β1-42 polymerization by fluorescence correlation spectroscopy
著者
Miwa, Koji Department of Environmental Physiology, Shimane University Faculty of Medicine, Shimane, Japan
橋本 道男
Shahdat Hossain Department of Environmental Physiology, Shimane University Faculty of Medicine, Shimane, Japan
片倉 賢紀
内容記述(抄録等)
Amyloid β (Aβ)1-42 fibrillation is a crucial step in the development of pathological hallmarks, such as neuritic plaques and neurofibrillary tangles, of Alzheimer’s disease (AD). In this study, we evaluated the effects of free docosahexaenoic acid (DHA), an essential brain polyunsaturated fatty acid (PUFA), on the inhibition of Aβ1-42 fibrillation by fluorescence correlation spectroscopy (FCS), a technique capable of detecting molecular movements and interactions in solution. We also examined whether free arachidonic acid (AA), eicosapentaenoic acid (EPA), and metabolites of DHA, including neuroprotectin D1 (NPD1, 10S, 17S-dihydroxy-DHA), resolvin D1 (RvD1, 7S, 8R, 17S-trihydroxy-DHA), and didocosahexaenoyl glycerol (diDHA), affect Aβ1-42 polymerization. The results of the FCS study reveal that DHA and AA significantly reduced the diffusion time of TAMRA (5-carboxytetramethylrhodamine)-Aβ1-42 by 28% and 31%, respectively, while EPA, NPD1, RvD1, and diDHA had no effects on diffusion time. These results indicate that DHA and AA inhibited Aβ1-42 polymerization and that their inhibitory effects occurred at the initial stage of Aβ1-42 polymerization. This study will advance the research on PUFAs in preventing AD progression.
主題
Docosahexaenoic Acid
Arachidonic Acid
Fluorescence Correlation Spectroscopy
Amyloid β Peptide
Fibrillation
掲載誌名
Advances in Alzheimer's Disease
2
2
開始ページ
66
終了ページ
72
DOI
出版者
Scientific Research Publishing Inc.
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
出版社版
業績ID
e16802
部局
医学部
このエントリーをはてなブックマークに追加