ダウンロード数 : ?
ID 38264
ファイル
言語
英語
著者
Florenza A. La Spina
Lis C. Puga Molina
Ana Romarowski
Alejandra M. Vitale
Tomas L. Falzone
Dario Krapf
Mariano G. Buffone
内容記述(抄録等)
Recent evidence demonstrated that most fertilizing mouse sperm undergo acrosomal exocytosis (AE) before binding to the zona pellucida of the eggs. However, the sites where fertilizing sperm could initiate AE and what stimuli trigger it remain unknown. Therefore, the aim of this study was to determine physiological sites of AE by using double transgenic mouse sperm, which carried EGFP in the acrosome and DsRed2 fluorescence in mitochondria. Using live imaging of sperm during in vitro fertilization of cumulus-oocyte complexes, it was observed that most sperm did not undergo AE. Thus, the occurrence of AE within the female reproductive tract was evaluated in the physiological context where this process occurs. Most sperm in the lower segments of the oviduct were acrosome-intact; however, a significant number of sperm that reached the upper isthmus had undergone AE. In the ampulla, only 5% of the sperm were acrosome-intact. These results support our previous observations that most of mouse sperm do not initiate AE close to or on the ZP, and further demonstrate that a significant proportion of sperm initiate AE in the upper segments of the oviductal isthmus.
主題
Sperm
Acrosomal exocytosis
Oviduct
Fertilization
Acrosome
掲載誌名
Developmental biology
411
2
開始ページ
172
終了ページ
182
ISSN
00121606
発行日
2016-03-15
DOI
DOI公開日
2016-12-14
NCID
AA00627848
出版者
Elsevier
資料タイプ
学術雑誌論文
ファイル形式
PDF
権利関係
Copyright © 2016 Elsevier Inc. All rights reserved.
著者版/出版社版
著者版
業績ID
e29541
部局
生物資源科学部 附属生物資源教育研究センター