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language
eng
Author
Miyazaki, Shoya Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan
Description
The endosymbiosis between Paramecium bursaria and Chlorella spp. is mutualistic. Symbiotic algae localize beneath the host Paramecium cell cortex compete for their attachment sites with preexisting organelle trichocysts. To examine the relationship between P. bursaria trichocysts and their symbiotic algae, algae-bearing or alga-free P. bursaria were starved for several days and the changes in the number of Chlorella sp. and presence or absence of trichocysts were evaluated. We conducted an indirect immunofluorescence microscopy with an anti-trichocyst monoclonal antibody against P. bursaria cells. Indirect immunofluorescence microscopy demonstrated that under starvation and darkness conditions, the immunofluorescence of trichocysts in alga-free P. bursaria decreased much faster than that in the normal algae-bearing P. bursaria. In the latter case, our observations proposed the possibility that the nutrition obtained from symbiotic algal digestion may promote trichocysts synthesis. This algal digestion mechanism may permit host P. bursaria cells to survive for a longer time under starvation condition. To the best of our knowledge, this may be a new benefit that host P. bursaria gain from harboring symbiotic algae.
Journal Title
Current Microbiology
Volume
78
Start Page
558
End Page
565
ISSN
0343-8651
ISSN(Online)
1432-0991
Published Date
2021-01-03
DOI
Publisher
Springer
NII Type
Journal Article
Format
PDF
Relation
Text Version
著者版
OAI-PMH Set
Faculty of Life and Environmental Science