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Title Transcription
ドジョウユライセイ サイキン ノ セイサンスル チアミナーゼ 1 ノ セイセイ ト ソノ コウソガクテキ セイシツ
Title Alternative (English)
Purification and Enzymological Properties of the Bacterial Thiaminase I Produced by a Stain Isolated from Soil
File
language
jpn
Author
Mochida, Kazuo
Ozoe, Yoshihisa
Fujita, Keiji
Suzuki, Kiroku
Description
Purification and enzymological properties of the bacterial thiaminase I produced by B. thiaminolyticus Strain, B isolated from soil has been described.
By applying the culture filtrate of the bacteria to ammonium sulfate fractionation and chromatographic fractionation using a DEAE-Sephadex A-50, Sephadex G-100(1st), Hydroxyl apatite, and Sephadex G-100(2nd), an enzyme preparation having ca. 260 times as much specific activity as that of the original crude enzyme was obtained. The enzyme was homogeneous on polyacrylamide disc gel electrophoresis and had a molecular weight, estimated by gel filtration, of 37,000 daltons. The optimum pH and the optimum temperature were 5.0 and 50℃ respectively. The metal ions such as <Cu>^<2+>, <Ag>^+, and <Hg>^<2+>, and PCMB remarkably inhibited the activity of the enzyme.
The kinetic parameters for the base-exchange reaction of the enzyme were determined at pH 5.0 and 50℃. The Km values for thiamine and pyridine were 2.7 and 12.8mM, respectively, and the V_max value was 120mg/mg protein/mm, expressed as the amount of thiamine decomposed.
These properties were compared with those of thiaminases from B. thiaminolyticus Matsukawa et Misawa and Cl. sporogenes.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
15
Start Page
131
End Page
137
ISSN
0370940X
Published Date
1981-12-15
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science
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