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language
eng
Author
Nishino, Kohei Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan
Kushima, Misaki Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan
Description
Cell lysis is induced in Schizosaccharomyces pombe ∆ura4 cells grown in YPD medium, which contains yeast extract, polypeptone, and glucose. To identify the medium components that induce cell lysis, we first tested various kinds of yeast extracts from different suppliers. Cell lysis of ∆ura4 cells on YE medium was observed when yeast extracts from OXOID, BD, Oriental, and Difco were used, but not when using yeast extract from Kyokuto. To determine which compounds induced cell lysis, we subjected yeast extract and polypeptone to GC-MS analysis. Ten kinds of compounds were detected in OXOID and BD yeast extracts, but not in Kyokuto yeast extract. Among them was urea, which was also present in polypeptone, and it clearly induced cell lysis. Deletion of the ure2 gene, which is responsible for utilizing urea, abolished the lytic effect of urea. The effect of urea was suppressed by deletion of pub1, and a similar phenotype was observed in the presence of polypeptone. Thus, urea is an inducer of cell lysis in S. pombe ∆ura4 cells.
Subject
cell lysis
urea
fission yeast
UMP de novo synthesis
ura4
Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume
81
Issue
7
Start Page
1444
End Page
1451
ISSN
0916-8451
ISSN(Online)
1347-6947
Published Date
2017-3-27
DOI
Publisher
公益社団法人日本農芸化学会
NII Type
Journal Article
Format
PDF
Text Version
著者版
Gyoseki ID
e33495
OAI-PMH Set
Faculty of Life and Environmental Science
Remark
This is an original manuscript / preprint of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2017-3-27, available online: http://www.tandfonline.com/10.1080/09168451.2017.1303360