File
language
eng
Author
Sheikh, Abdullah Md.
Ochi, Hiroshi
Masuda, Junichi
Description
Lysophosphatidylcholine (lysoPC), a major phospholipid component of oxidized LDL, changes the expression of multiple genes in endothelial cells leading to endothelial dysfunction, and plays an important role in the development of atherosclerosis. We performed a gene expression analysis to identify novel lysoPC-inducible genes in endothelial cells; using macroarray analysis as a screening tool and real time PCR to verify the result of the macroarray. Using this approach, we demonstrated that the mRNA expression of Rac-2, Rab-11, integrin α6 and integrin β4 was upregulated by lysoPC in endothelial cells. The analysis of the mRNA expression profile revealed that lysoPC induced those mRNA expressions in time and concentration dependent manners. Flow cytometric analysis showed that lysoPC selectively increased the surface expression of integrin α6 and integrin β4 on endothelial cells. Thus, we provided an evidence that lysoPC upregulated four genes belonging to the small G protein and integrin family at the mRNA Ievel, and at least integrin α6 and integrin β4 at the protein level in endothelial cells, which might implicate the substantial involvement of lysoPC in the phenotypic changes of endotherial cells.
Journal Title
Shimane journal of medical science
Volume
22
Issue
2
Start Page
19
End Page
28
ISSN
03865959
ISSN(Online)
24332410
Published Date
2005-12-01
NCID
AA00841586
Publisher Aalternative
Shimane University Faculty of Medicine
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
Remark
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html