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Title Transcription
リョウセイルイ オヨビ ホニュウルイ バイヨウ サイボウ ノ ブンレツ ジ ニ オケル ジン ノ ケンシュツ
Title Alternative (English)
Cyiochemical Studies for Demonstration oi Nucleoli in Mitotic Divisions of Amphibian and Mammalian Cells in Vitro
File
language
eng
Author
Seto, Takeshi
Description
Cytochemical methods proposed for the demonstration of nucleoli were comparatively studied in order to obtain more specific visualization of nucleoli with a light microscope.
The amphibian cell culture derived from frog kidney tissue was successfully used in stain preparations of mitotic cells, and some modifications of staining methods resulted effective manifestation of nucleoli at mitotic phases.
Moderate extraction of RNA from fixed cells with perchloric acid or RNase was recognized more effective to the subsequent nucleolar stainings for the detection of persistent nucleoli in any kind of cultured cells.
Journal Title
Memoirs of the Faculty of Education, Shimane University. Natural science
Volume
8
Start Page
29
End Page
36
ISSN
05869943
Published Date
1974-12-25
NCID
AN00107941
Publisher
島根大学教育学部
Publisher Aalternative
The Faculty of Education Shimane University
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Education
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