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language
eng
Attribute
Original article
Author
GONG, Ao
JING, Li
Description
Type I collagen, which consists of two α1 and one α2 chains, is an abundant extracellular matrix ( ECM ) protein. Changes in type I collagen are often associated with various diseases including fibrosis, osteogenesis imperfecta ( OI ), and Ehlers-Danlos syndrome ( EDS ). In the present study, we developed a method for quantification of type I collagen α1 ( COL1A1 ) in a culture medium of LX-2 human hepatic stellate cells by using nano-liquid chromatography tandem mass spectrometry ( nano-LC/MS/MS ). After selecting a specific peptide of COL1A1, unlabeled and stable isotope-labeled peptides were used for quantitative analysis of COL1A1 by nano-LC/MS/MS. The concentration of secreted COL1A1 in the LX-2 cell culture medium was 38.78 ng/mL. The results indicate that this method is useful for quantifying COL1A1 in a cell culture medium.
Subject
type I collagen
COL1A1
nano-LC/MS/ MS
stable isotope-labeled peptide
Journal Title
Shimane Journal of Medical Science
Volume
38
Issue
1
Start Page
1
End Page
5
ISSN
03865959
ISSN(Online)
24332410
Published Date
2021-03
NCID
AA00841586
Publisher
Faculty of Medicine, Shimane University
Publisher Aalternative
島根大学医学部
NII Type
Departmental Bulletin Paper
Format
PDF
Rights
Faculty of Medicine, Shimane University
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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