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language
eng
Author
Ishikawa, Satoru
Matsui, Yasunori
Wachi, Satoshi
Yamaguchi, Hiroshi
Harashima, Nanae
Description
Because cancer is associated with aging, immunological features in the aged should be considered in anticancer immunotherapy. In this study, we investigated antitumor immunity in aged mice using a CT26 colon carcinoma model. The tumor growth of CT26 was accelerated in aged mice compared with that in young mice, but this difference was not observed in nude mice. The serum levels of IL-6 and TNF-α were higher in aged mice than those in young mice, irrespective of the CT26-bearing state. The in vitro induction of CT26-specific CTLs from aged mice that were vaccinated with doxorubicin (DTX)-treated CT26 cells was impaired. In vivo neutralization of IL-6, but not TNF-α, showed a tendency to restore the in vitro induction of CT26-specific CTLs from vaccinated aged mice. Analyses on tumor-infiltrating immune cells as early as day 5 after CT26 inoculation revealed that monocytic and granulocytic MDSCs preferentially infiltrated into tumor sites in aged mice compared with young mice. Alternatively, oral administration of Lentinula edodes mycelia (L.E.M.) extract, which has the potential to suppress inflammation in tumor-bearing hosts, decreased the serum levels of IL-6 in aged mice. When administration of L.E.M. extract was started 1 week earlier, CT26 growth was retarded in aged mice and the in vivo priming of tumor-specific CTLs was improved in CT26-vaccinated aged mice. These results indicate early infiltration of MDSCs is related to impaired immunity of aged hosts and that oral administration of L.E.M. extract can mitigate the impairment.
Subject
Aging
Inflammation
IL-6
MDSC
T cell immunity
Journal Title
Cancer Immunology and Immunotherapy
Volume
65
Issue
8
Start Page
961
End Page
972
ISSN
03407004
Published Date
2016-08
DOI
DOI Date
2017-03-15
PubMed ID
NCID
AA00598499
Publisher
Springer
NII Type
Journal Article
Format
PDF
Rights
© Springer-Verlag Berlin Heidelberg 2016
Text Version
著者版
OAI-PMH Set
Faculty of Medicine