number of downloads : ?
File
language
eng
Author
Yoshida, Hiroshi
Description
Kinetic constants toward high molecular weight RNA have been determined with native ribonuclease F_1 and the enzyme acetylated at lysine 62. The Michaelis constant and the maximum velocity are 0.75 mg/ml and 0.32 U/nM, respectively, with ribonuclease F_1 and 0.93 mg/ml and 0.13 U/nM, respectively, with the acetylated enzyme. These results suggest that lysine 62 does not constitute a phosphate binding subsite, but somehow participates in the catalytic breakdown of high molecular weight substrates.
Subject
ribonuclease F_1
acetylation
enzyme kinetics
Journal Title
Bulletin of Shimane Medical University
Volume
15
Start Page
87
End Page
90
ISSN
03879097
Published Date
1992-12-01
NCID
AN00107602
Publisher
島根医科大学
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
他の一覧