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Title Transcription
スイコウ サイバイ キュウリ ノ バイヨウ エキ カラ ブンリ シタ フェノール ブッシツ ガ カジツ シュウリョウ ニ オヨボス エイキョウ
Title Alternative (English)
Influences of Phenolics Isolated from the Nutrient Solution Nourishing Growing Cucumber (Cucumis sativus L.) Plants on Fruit Yield
Author
Md. H. R. Pramanik Faculty of Life and Environmental Science, Shimane University
Tomita, Kouhei Faculty of Life and Environmental Science, Shimane University
Ohba, Yumiko Faculty of Life and Environmental Science, Shimane University
Hosoki, Takashi
Matsui, Yoshihisa Faculty of Life and Environmental Science, Shimane University
Description
水耕キュウリの培養液から単離された生長抑制物質について検討を行った.培養液に活性炭を加え, 生長抑制物質を吸着させ, 活性炭から抑制物質を有機溶媒により溶出し, 4分画に分別した.1.キュウリに対する生長抑制は酸性のジエチルエーテル可溶性分画にみられたが, 他の分画ではみられなかった.2. 上記の活性分画のGC/MS法により検索したところ, benzoic acid, p-hydroxybenzoic acid, 2, 4-dichlorobenzoic acidおよびphthalic acidが同定された.3. 前述の種類の物質を各種濃度で培養液に添加し, キュウリ幼苗に対する生長抑制活性を調査した.その結果, 2, 4-dichlorobenzoic acidが生長を最も抑制したが, 他の物質の生長抑制活性は極めて弱かった.4. 培養液に2, 4-dichlorobenzoic acidを2 μmol・liter-1添加すると, 栄養生長には影響はみられなかったが, 果実収量は低下し, 果実の収穫期間も短くなる影響がみられた.この影響は培養液への活性炭添加により除去された.5. 以上の結果から, 2, 4-dichlorobenzoic acidは水耕栽培キュウリにおいて生長抑制効果があり, 本成分は活性炭に吸着除去できることが明らかにされた.
Description Alternative
Abstract
Growth inhibiting substances of unknown origin found in the nutrient solution nourishing growing cucumber plants were isolated and identified. The growth inhibitors were absorbed on activated charcoal and extracted therefrom by an organic solvent. These extracts were partitioned into four fractions with different solvents. 1. The inhibiting activity for cucumber plants was detected in the acidic diethyl ether soluble fraction, but not in others. 2. The active substances in the above fraction analyzed by GC/MS method were identified as : benzoic acid, p-hydroxybenzoic acid, 2, 4-dichlorobenzoic acid and phthalic acid. 3. The above substances were assayed for their activity on cucumber seedlings by adding various concentrations to the nutrient solution. 2, 4-dichlorobenzoic acid exhibited the strongest inhibitory activity ; others were very weak. 4. 2, 4-dichlorobenzoic acid at 2 μmol·liter-1 in nutrient solution had no effect on plant growth but it lowered fruit yield and shortened fruit harvesting period, compared with the control. Any inhibition was eliminated by adding activated charcoal to the nutrient solution. 5. Thus, 2, 4-dichlorobenzoic acid, the most effective inhibitor in the nutrient solution nourishing growing cucumber plants can be eliminated by adding activated charcoal.
Subject
activated charcoal
autotoxicity
cucumber
fruit yield
hydroponics
2, 4-dichlorobenzoic acid
Journal Title
Journal of the Japanese Society for Horticultural Science
Volume
68
Issue
4
Start Page
847
End Page
853
ISSN
0013-7626
ISSN(Online)
1880-358X
Published Date
1999
DOI
Publisher
園芸学会
Publisher Transcription
エンゲイ ガッカイ
Publisher Aalternative
Japanese Society for Horticultural Science
NII Type
Journal Article
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Life and Environmental Science