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language
eng
Author
MATSUO, Yuzy Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
KISHIMOTO, Hayafumi Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
HORIUCHI, Tomitaka Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
TANAE, Katsuhiro Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
Description
Gap-repair cloning for plasmid construction in budding yeast is very effective and often used. In contrast, the same method is not widely used in fission yeast, because of a shortage of information on it. Here we describe simple and effective gap-repair cloning for plasmid construction using short tracts of flanking homology. By this method, we combined concentrated DNA fragments with short (20 bp) tracts of flanking homology with the marker gene or the pre-existing gene module. In addition, we found that this method can be applied to one-step cloning of multiple DNA fragments to construct a fusion gene.
Subject
fission yeast
gap-repair cloning
short tracts of flanking homology
one-step cloning of multiple DNA fragments
Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume
74
Issue
3
Start Page
685
End Page
689
ISSN
0916-8451
ISSN(Online)
1347-6947
Published Date
2010-03-23
DOI
Publisher
Japan Society for Bioscience, Biotechnology, and Agrochemistry
NII Type
Journal Article
Format
PDF
Text Version
著者版
OAI-PMH Set
Faculty of Life and Environmental Science