number of downloads : ?
File
language
eng
Title Alternative (English)
Evaluation of the inhibitory effect of docosahexaenoic acid and arachidonic acid on the initial stage of amyloid β1-42 polymerization by fluorescence correlation spectroscopy
Author
Miwa, Koji Department of Environmental Physiology, Shimane University Faculty of Medicine, Shimane, Japan
Hashimoto, Michio
Shahdat Hossain Department of Environmental Physiology, Shimane University Faculty of Medicine, Shimane, Japan
Katakura, Masanori
Description
Amyloid β (Aβ)1-42 fibrillation is a crucial step in the development of pathological hallmarks, such as neuritic plaques and neurofibrillary tangles, of Alzheimer’s disease (AD). In this study, we evaluated the effects of free docosahexaenoic acid (DHA), an essential brain polyunsaturated fatty acid (PUFA), on the inhibition of Aβ1-42 fibrillation by fluorescence correlation spectroscopy (FCS), a technique capable of detecting molecular movements and interactions in solution. We also examined whether free arachidonic acid (AA), eicosapentaenoic acid (EPA), and metabolites of DHA, including neuroprotectin D1 (NPD1, 10S, 17S-dihydroxy-DHA), resolvin D1 (RvD1, 7S, 8R, 17S-trihydroxy-DHA), and didocosahexaenoyl glycerol (diDHA), affect Aβ1-42 polymerization. The results of the FCS study reveal that DHA and AA significantly reduced the diffusion time of TAMRA (5-carboxytetramethylrhodamine)-Aβ1-42 by 28% and 31%, respectively, while EPA, NPD1, RvD1, and diDHA had no effects on diffusion time. These results indicate that DHA and AA inhibited Aβ1-42 polymerization and that their inhibitory effects occurred at the initial stage of Aβ1-42 polymerization. This study will advance the research on PUFAs in preventing AD progression.
Subject
Docosahexaenoic Acid
Arachidonic Acid
Fluorescence Correlation Spectroscopy
Amyloid β Peptide
Fibrillation
Journal Title
Advances in Alzheimer's Disease
Volume
2
Issue
2
Start Page
66
End Page
72
DOI
Publisher
Scientific Research Publishing Inc.
NII Type
Journal Article
Format
PDF
Text Version
出版社版
Gyoseki ID
e16802
OAI-PMH Set
Faculty of Medicine