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language
eng
Author
Kobayashi, Yuta
TAKEUCHI, Takashi Course of Veterinary Laboratory Medicine, Faculty of Agriculture, Tottori University, 4-101 Minami, Koyama-cho, Tottori 680-8533, Japan
Description
The anesthetic mixture of medetomidine (MED), midazolam (MID) and butorphanol (BUT) produced anesthetic duration of around 40 minutes (min) in ICR mice. We reported that this anesthetic mixture produced almost the same anesthetic effects in both male and female BALB/c and C57BL/6J strains. Intraperitoneal (IP) administration of drugs has been widely used in mice. However, various injectable routes of the anesthetic mixture may cause different anesthetic effects. First, we examined effects of the anesthetic mixture by subcutaneous (SC) and intravenous (IV) injection compared to IP injection. After injection of the anesthetic mixture, administration of atipamezole (ATI) induced mice recovery from anesthesia. Secondly, we examined how different dosage and optimum injection timing of ATI affected mice recovery from anesthesia. We used an anesthetic score to measure anesthetic duration and a pulse oximeter to monitor vital signs under anesthesia. Usually, drugs from SC injection work more weakly than IP or IV injection. However, we found no significant differences of anesthetic duration among the three different injection routes. Antagonistic effects of ATI (0.3 mg/kg and 1.5 mg/kg) worked equally when administered at 30 min after injection of the anesthetic mixture. Antagonistic effects of ATI (1.5 mg/kg) were stronger than ATI (0.3 mg/kg) at 10 min after injection of the anesthetic mixture. The anesthetic mixture is a useful drug to induce nearly the same anesthetic effects by different injection routes and has an antagonist of ATI which helps mice quickly recover from anesthesia. These results may contribute to the welfare of laboratory animals.
Subject
anesthetic mixture
antagonist
atipamezole
mice
pulse oximeter
Journal Title
Experimental Animals
Volume
64
Issue
1
Start Page
39
End Page
47
ISSN
1341-1357
ISSN(Online)
1881-7122
Published Date
2015
DOI
Publisher
公益社団法人 日本実験動物学会
Publisher Transcription
コウエキ シャダン ホウジン ニホン ジッケン ドウブツ ガッカイ
NII Type
Journal Article
Format
PDF
Text Version
出版社版
Gyoseki ID
e26057
OAI-PMH Set
Faculty of Medicine