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language
eng
Author
Yoshimatsu, K Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843, Japan.
Kawahara, N Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843, Japan.
Yamamoto, S.-I. National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan.
Niino, T. Gene Research Center, University of Tsukuba, Tsukuba 305-8572, Japan.
Description
A clonal propagation method by node culture of perilla (Perilla frutescens L. Britton) was investigated. Nodes were plated on solidified 1/2 MS medium with BA and optimum shoot elongation and propagation was obtained at BA 0.05 and 0.1 mg l-1. Cryopreservation using an aluminum cryo-plate was successfully applied to in vitro-grown perilla shoot tips. Excised shoot tips from nodes were precultured on 1/2 MS medium with 0.3 M sucrose and embedded on an aluminum cryo-plate with alginate gel. The cryo-plate with shoot tips was osmo-protected with LS solution and dehydrated in PVS2 for 20 min at 25°C prior to immersion into liquid nitrogen. The recovery growth after cryopreservation was found to be about 80%. This new V-Cryo-plate method has many advantages and may facilitate the cryo-storage of other medicinal plants.
Subject
Cryopreservation
Aluminum
Regrowth
Vitrification
Plants
Clonal propagation
Hypocotyls
Chromosomes
Plant genetics
Japanese culture
Journal Title
Advances in Horticultural Science
Volume
28
Issue
2
Start Page
79
End Page
83
Published Date
2014
DOI
Publisher
Dipartimento Di Scienze Delle Produzioni Vegetali, Del Suolo E Dell'Ambiente Agroforestale – DiPSA – University of Florence
NII Type
Journal Article
Format
PDF
Relation
Text Version
出版社版
Gyoseki ID
e26030
OAI-PMH Set
Faculty of Life and Environmental Science