Tanae, Katsuhiro Department of Appl ied Bioscience and Biotechnology, Facul ty of Li fe and Environmental Science, Shimane Universi ty, Matsue, 690-8504, Japan
Horiuchi, Tomitaka Department of Appl ied Bioscience and Biotechnology, Facul ty of Li fe and Environmental Science, Shimane Universi ty, Matsue, 690-8504, Japan
Matsuo, Yuzy Department of Appl ied Bioscience and Biotechnology, Facul ty of Li fe and Environmental Science, Shimane Universi ty, Matsue, 690-8504, Japan
An H3/H4 histone chaperone, Asf1, plays an essent ial role in maintaining genomic stabil i ty in many species, including f ission yeast . Here, we showed that overexpression of a CENP-A chaperone Sim3 suppres sed the temperature sensi t i ve phenotype of asf1-33 and asf1-30 mutants and the defect in chromat in st ructure, and prevented the accumulat ion of DNA damage in asf1-33 mutants at high temperatures. Fur thermore, asf1-33 and Δsim3 were synthet ic lethal . Consistent with this, shutdown of s im3 expression in asf1-33 Δsim3 double mutants that contained extragenic s im3 resul ted in growth retardat ion. In addi t ion, the Δs im3 mutant displayed sens i t ivi ty to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin st ructur e . Our resul ts sugges t a possibil i ty that Sim3 funct ions as a histone chaperone.
the Federation of European Biochemical Societies Edited by: Felix Wieland
Faculty of Life and Environmental Science
This is the peer reviewed version of the following article: https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2012.10.020, which has been published in final form at https://doi.org/10.1016/j.febslet.2012.10.020. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.