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language
eng
Author
Tie-Zhong Cui
Tomohiro Kaino
Description
The length of the isoprenoid-side chain in ubiquinone, an essential component of the electron transport chain, is defined by poly-prenyl diphosphate synthase, which comprises either homomers (e.g., IspB in Escherichia coli) or heteromers (e.g., decaprenyl diphosphate synthase (Dps1) and D-less polyprenyl diphosphate synthase (Dlp1) in Schizosaccharomyces pombe and in humans). We found that expression of either dlp1 or dps1 recovered the thermo-sensitive growth of an E. coli ispBR321A mutant and restored IspB activity and production of Coenzyme Q-8. IspB interacted with Dlp1 (or Dps1), forming a high-molecular weight complex that stabilized IspB, leading to full functionality.
Subject
Isoprenoid
Ubiquinone
Coenzyme Q
Prenyl diphosphate synthase
IspB
Journal Title
FEBS Letters
Volume
584
Issue
4
Start Page
652
End Page
656
ISSN
0014-5793
Published Date
2010-2-19
DOI
Publisher
Federation of European Biochemical Societies
NII Type
Journal Article
Format
PDF
Text Version
著者版
Gyoseki ID
e9311
OAI-PMH Set
Faculty of Life and Environmental Science