ダウンロード数 : ?
language
eng
Author
Haque, Md. Ahsanul
Hashimoto, Michio
Description
Mesenchymal stem cell transplantation is demonstrated to improve neurological performance in neurodegenerative diseases including Alzheimer’s disease. To understand the underlying mechanism, we infused amyloid β (Aβ) peptide in the lateral ventricle of adult Wister rats using osmotic pump. After 15 days of continuous infusion, a mesenchymal stem cell line (B10) was transplanted in the lateral ventricle. Learning related behavior was evaluated by 2-way shuttle avoidance test. The results showed that Aβ infusion significantly decreased learning related behavior, whereas that was not decreased in B10 transplanted group. Nissl staining results demonstrated that hippocampal pyramidal neurons in CA1 area was decreased in Aβ-infused rats, whereas that was not decreased in B10 transplanted group. Immunohistochemical examination showed that Aβ mainly deposited in the vessels of the brains of Aβ-infused rats, which was decreased by B10 transplantation. Further analysis showed that B10 transplantation increased vessel density, as revealed by immunofluorescence staining. Moreover, the number of astrocyte and microglia was decreased by Aβ infusion, which was returned to the level of sham animals by B10 transplantation. Real time PCR and immunostaining results showed that B10 transplantation significantly increased IL-1β mRNA and protein expression. Thus, our result showed that MSC transplantation effectively decreased Aβ deposition in cerebral vessel and increased angiogenesis, which could be possible cause of improved neurological performance in Aβ-infused rats.
Subject
Mesenchymal stem cell
Alzheimer’s disease
amyloid β protein
angiogenesis
Journal Title
Current Alzheimer Research
Volume
15
Issue
14
Start Page
1331
End Page
1342
ISSN
1567-2050
ISSN(Online)
1875-5828
Published Date
2018
DOI
PubMed ID
Publisher
Saif Zone, Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, c2004-
NII Type
Journal Article
Format
PDF
Text Version
著者版
OAI-PMH Set
Faculty of Medicine
Diploma Number
甲第663号
Granted Date
2019-03-22
Degree Name
博士(医学)
Grantor
島根大学
Graduate Course
Graduate School of Medical Research
Granted Year
2018
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