ID 36677
File
language
eng
Author
Florenza A La Spina
Ana Romarowski
Mariano G Buffone
Description
Mammalian spermatozoa must undergo complex physiological and morphological alterations within the female reproductive tract before they become fertilization competent. Two important alterations are capacitation and the acrosome reaction (AR), by which spermatozoa become capable of penetrating the zona pellucida (ZP) of the oocyte. Although various biochemical stimulants have been reported to induce the AR, the true physiological inducer in vivo remains to be identified. Previously, it has been reported that most fertilizing spermatozoa undergo the AR before contacting the ZP and that only a small fraction of in vitro-capacitated spermatozoa can penetrate the ZP. Therefore, it is important to identify which capacitating spermatozoa undergo the AR in response to potential AR inducers such as progesterone. Here we show that spermatozoa undergo a dynamic rearrangement of the acrosome during in vitro capacitation. This involves the rapid movement of an artificially introduced soluble component of the acrosome, enhanced green fluorescent protein (EGFP), from the acrosomal cap region to the equatorial segment (EQ) of the sperm head. Spermatozoa exhibiting the EQ pattern were more sensitive to progesterone than were those without it. We suggest that spermatozoa that are ready to undergo acrosomal exocytosis can be detected by real-time EGFP imaging. This offers a promising new method for identifying where spermatozoa undergo the AR in the female reproductive tract in vivo.
Journal Title
Reproduction
Volume
149
Start Page
657
End Page
663
ISSN
14701626
Published Date
2015-04-01
DOI
DOI Date
2016-12-14
NCID
AA11501251
Publisher
Society for the Study of Fertility
NII Type
Journal Article
Format
PDF
Rights
© 2015 Society for Reproduction and Fertility
Disclaimer: this is not the definitive version of record of this article.This manuscript has been accepted for publication in 'Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/REP-15-0017 2015.
Text Version
著者版
Gyoseki ID
e29540
OAI-PMH Set
Education and Research Center for Biological Research, Faculty of Life and Environmental Science