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ファイル
言語
英語
著者
Fujishima, Masahiro
内容記述(抄録等)
Chlorella variabilis strain NC64A is an intracellular photobiont of the ciliate Paramecium bursaria. NC64A was isolated from P. bursaria nearly 50 years ago and was thereafter cultivated outside the host. This study was undertaken to detect changes in its infectivity to P. bursaria and its auxotrophy for growth outside the host induced during long-term cultivation. NC64A can grow in Modified Bold's Basal Medium but not in C medium, whereas another symbiotic Chlorella variabilis strain, 1N, that was recently isolated from the host grew in C medium but not in Modified Bold's Basal Medium. With regards infectivity, NC64A in the logarithmic phase of growth showed low infectivity to alga-removed P. bursaria cells, whereas those in the early stationary phase showed high infectivity of about 30%. Those in the decay phase of growth showed no infectivity. Results show that NC64A has infectivity, but the infection rate depends on their culture age in the growth curve. Furthermore, NC64A that had been re-infected to P. bursaria for more than one year and isolated from the host showed a nearly 100% infection rate, which indicates that NC64A can recover its infectivity by re-infection to P. bursaria.
主題
Auxotrophy
Chlorella variabilis
Endosymbiosis
Infectivity
Long-term cultivation
Paramecium bursaria
掲載誌名
Biology open
5
1
開始ページ
55
終了ページ
61
ISSN
20466390
発行日
2016-01-15
DOI
出版者
Company of Biologists
資料タイプ
学術雑誌論文
ファイル形式
PDF
権利関係
© 2015. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
著者版/出版社版
出版社版
業績ID
e29438
部局
生物資源科学部