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ファイル
言語
英語
著者
Florenza A La Spina
Ana Romarowski
Mariano G Buffone
内容記述(抄録等)
Mammalian spermatozoa must undergo complex physiological and morphological alterations within the female reproductive tract before they become fertilization competent. Two important alterations are capacitation and the acrosome reaction (AR), by which spermatozoa become capable of penetrating the zona pellucida (ZP) of the oocyte. Although various biochemical stimulants have been reported to induce the AR, the true physiological inducer in vivo remains to be identified. Previously, it has been reported that most fertilizing spermatozoa undergo the AR before contacting the ZP and that only a small fraction of in vitro-capacitated spermatozoa can penetrate the ZP. Therefore, it is important to identify which capacitating spermatozoa undergo the AR in response to potential AR inducers such as progesterone. Here we show that spermatozoa undergo a dynamic rearrangement of the acrosome during in vitro capacitation. This involves the rapid movement of an artificially introduced soluble component of the acrosome, enhanced green fluorescent protein (EGFP), from the acrosomal cap region to the equatorial segment (EQ) of the sperm head. Spermatozoa exhibiting the EQ pattern were more sensitive to progesterone than were those without it. We suggest that spermatozoa that are ready to undergo acrosomal exocytosis can be detected by real-time EGFP imaging. This offers a promising new method for identifying where spermatozoa undergo the AR in the female reproductive tract in vivo.
掲載誌名
Reproduction
149
開始ページ
657
終了ページ
663
ISSN
14701626
発行日
2015-04-01
DOI
DOI公開日
2016-12-14
NCID
AA11501251
出版者
Society for the Study of Fertility
資料タイプ
学術雑誌論文
ファイル形式
PDF
権利関係
© 2015 Society for Reproduction and Fertility
Disclaimer: this is not the definitive version of record of this article.This manuscript has been accepted for publication in 'Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/REP-15-0017 2015.
著者版/出版社版
著者版
業績ID
e29540
部局
生物資源科学部 附属生物資源教育研究センター
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