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タイトルヨミ
ベイシツ ニ カンスル ケンキュウ ダイ3ポウ ロシ デンキ エイドウホウ ニヨル ベイリュウ タンパク ノ ケンサク
日本語以外のタイトル
Studies on the Characters of Rice Kernel-"Beisitu"(3) : Detection of the Protein by mean of the Paperelectrophoresis in Rice Kernels
ファイル
言語
日本語
著者
高野 圭三
野津 幹雄
西川 省造
内容記述(抄録等)
The present paper deals with investigations on the changes of water soluble proteins of the rice kernels in relation to the changes in ripening and during storage.
The proteins were extracted from rice kernels (10g) with distilled water (30cc) and after centrifugation (4,000r.p.m.-10min.), the supernatant (25cc) was concentrated with Visking seamless cellulose tubing against polyethylen glycol-1,500. Given conditions for paperelectrophoresis were 0.5 mA/cm current and 15~20 V/cm voltage in barbiturate buffer (pH 8.6 ionic strength 0.05) and the changes of the optical densities in that pattern were measured by Hitachi's densitometer after staining with 1 % B.P.B. ethanol solution and 0.5 % acetic acid solution.
In younger fruits of rice, even at 7 days after heading, 2 peaks in the pattern were clearly divided, though in the leaf extract, 1 peak was only found. At final stage of ripening (brown rice), 4 peaks were observed in the electrophoretic pattern. So far as this experiment was concerend, the water soluble proteins seem to be composed of 4 proteins in endosperm and 2 proteins in embryo and these peaks seem to disappeare in later storage.
掲載誌名
島根農科大学研究報告
10
開始ページ
(A)1
終了ページ
4
ISSN
05598311
発行日
1962-03-31
NCID
AN00108241
出版者
島根農科大学
出版者別表記
The Shimane Agricultural College
資料タイプ
紀要論文
部局
生物資源科学部
備考
A,Bを含む
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