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Title Transcription
ダイコン シヨウ ノ ヨウリョクタイ ハッセイ ニ カンスル ケンキュウ 4 リンゴサン ダツ スイソ コウソ ニツイテ
Title Alternative (English)
Studies on the Chloroplast Development in Radish Cotyledons(4) : on Malate Dehydrogenases
File
language
jpn
Author
Ochiai, Hideo
Kono, Yasuhisa
Shibata, Hitoshi
Description
ocalization, properties and time courses of malate dehydrogenases in greening radish cotyledons were studied as well as an inhibitory effect of 4-thiouridine upon the enzymes. Results obtained are as follow.
1) Chloroplast fraction isolated from the radish cotyledon cells contained <NAD>^+ specific malate dehydrogenase, the specific activity of which decreased as the development of the chloroplast proceeded on illumination.
2) Non-chloroplast fraction contained <NADP>^+-malate dehydrogenase and malic enzyme. Cofactor of the <NADP>^+_malate dehydrogenase could be replaced by <NAD>^+ with a slight decrease of the specific activity. Malic enzyme required <NADP>^+ as a specific cofactor. Specific activities of both the enzymes remained unchanged with illumination time.
3) <NAD>^+_malate dehydrogenase of the chloroplast was inhibited by the presence of 4-thiouridine (1-5mM), but malic enzyme in the cytoplasm was not.
Based upon these observations, biological significance of <NAD>^+_malate dehydrogenase in the developing chloroplast was discussed.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
7
Start Page
129
End Page
134
ISSN
0370940X
Published Date
1973-12-15
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Faculty of Life and Environmental Science
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