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File
language
eng
Author
Ajiki, Masayuki
Description
A culture method for mouse embryos of post-implantation stage has not yet been established. In this study, we tried several methods of mouse embryo culture from blastocyst to early-somite stage using co-culture technique, human cord and/or rat sera and cytokines including leukemia inhibitory factor (LIF). Among feeder cells examined, placental cells appeared to secrete some factor (s) to promote post-implantation development. Among the sera examined, human cord serum supported best the post-implantation development. Although LIF seemed to promote pre-implantation development and implantation, it did not support post-implantation development. The present culture system is thus useful for observing embryos for two to four days after implantation. Using this system, Lim-1, a LIM class homeodomain-containing transcription factor, was observed in a part of the embryo and is suggested to play a role in early embryogenensis.
Journal Title
Shimane journal of medical science
Volume
17
Issue
2
Start Page
43
End Page
49
ISSN
03865959
ISSN(Online)
24332410
Published Date
1999-12-01
NCID
AA00841586
Publisher Aalternative
Shimane Medical University
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
Remark
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html
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