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language
eng
Author
Noothalapati, Hemanth Raman Center for Medical and Biological Applications, Shimane University
Uemura, Suguru Faculty of Life and Environmental Science, Shimane University
Ohshima, Naoki Raman Center for Medical and Biological Applications, Shimane University
Kinoshita, Yoshikazu Raman Center for Medical and Biological Applications, Shimane University
Ando, Masahiro Consolidated Research Institute for Advanced Science and Medical Care, Waseda University
Hamaguchi, Hiro-o Consolidated Research Institute for Advanced Science and Medical Care, Waseda University
Yamamoto, Tatsuyuki Raman Center for Medical and Biological Applications, Shimane University
Description
Eosinophilic esophagitis (EoE) is believed to be a rare pathological condition that is characterized by dense infiltration of eosinophils in esophageal epithelial layer. Occurrence of this disease worldwide has started increasing rapidly in the last decade. Routine endoscopic observations can help in diagnosis only if clear longitudinal furrows or multiple concentric rings are observed but does not give any definitive conclusion in the early stages. Hence esophageal tissue samples are collected from multiple sites by biopsy and the number of eosinophils is counted after staining. Such a procedure is time consuming and has an inherent risk of bleeding, eventually damaging esophagus. Hence we developed a resonance Raman spectroscopy based approach to detect eosinophils in esophagus using mouse models. Our results show the presence of eosinophils in mice esophageal tissues suffering from inflammation by administering interleukin-33 while there are none in control mice. We believe this method can be used in clinical application for diagnosing EoE in the near future.
Subject
Raman spectroscopy
Eosinophilic esophagitis
Non-bioptic diagnosis
Eosinophil peroxidase
Journal Title
Vibrational spectroscopy
Volume
85
Start Page
7
End Page
10
ISSN
09242031
Published Date
2016-07
DOI
DOI Date
2016-12-06
NCID
AA10787250
Publisher
Elsevier B.V.
NII Type
Journal Article
Format
PDF
Rights
© 2016 Elsevier B.V. All rights reserved.
Text Version
著者版
Gyoseki ID
e28856
e30205
OAI-PMH Set
Center for Promotion of Project Research
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science
School of Medicine, Faculty of Medicine
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