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File
language
eng
Author
Florenza A. La Spina
Lis C. Puga Molina
Ana Romarowski
Alejandra M. Vitale
Tomas L. Falzone
Dario Krapf
Mariano G. Buffone
Description
Recent evidence demonstrated that most fertilizing mouse sperm undergo acrosomal exocytosis (AE) before binding to the zona pellucida of the eggs. However, the sites where fertilizing sperm could initiate AE and what stimuli trigger it remain unknown. Therefore, the aim of this study was to determine physiological sites of AE by using double transgenic mouse sperm, which carried EGFP in the acrosome and DsRed2 fluorescence in mitochondria. Using live imaging of sperm during in vitro fertilization of cumulus-oocyte complexes, it was observed that most sperm did not undergo AE. Thus, the occurrence of AE within the female reproductive tract was evaluated in the physiological context where this process occurs. Most sperm in the lower segments of the oviduct were acrosome-intact; however, a significant number of sperm that reached the upper isthmus had undergone AE. In the ampulla, only 5% of the sperm were acrosome-intact. These results support our previous observations that most of mouse sperm do not initiate AE close to or on the ZP, and further demonstrate that a significant proportion of sperm initiate AE in the upper segments of the oviductal isthmus.
Subject
Sperm
Acrosomal exocytosis
Oviduct
Fertilization
Acrosome
Journal Title
Developmental biology
Volume
411
Issue
2
Start Page
172
End Page
182
ISSN
00121606
Published Date
2016-03-15
DOI
DOI Date
2016-12-14
NCID
AA00627848
Publisher
Elsevier
NII Type
Journal Article
Format
PDF
Rights
Copyright © 2016 Elsevier Inc. All rights reserved.
Text Version
著者版
Gyoseki ID
e29541
OAI-PMH Set
Education and Research Center for Biological Research, Faculty of Life and Environmental Science
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