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File
language
eng
Author
Xue, Yuing
Nakagami, Norihito
Imamura, Shinji
Kataoka, Kaori
Description
We have confirmed for the first time, both biochemically and immunologically, the existence of deoxyribonuclease I (DNase I) in the human tears. Isoelectric focusing of tear samples on polyacrylamide gels (pH 3.5-5), followed by dried agarose film overlay detection, was used to determine the phenotypes of tear DNase I. Because of its high degree of sensitivity and its high band resolution, this detection method allowed determination of DNase I types from tear samples of about 5 μL. Pretreatment of tear samples with the enzyme sialidase was essential for typing in order to markedly enhance the sensitivity and simplify the isozyme pattern. DNase I types in all tear samples were consistently related to types found in corresponding blood and urine samples. DNase I typing may, therefore, provide a novel discriminating characteristic in the genetic, clinical and forensic examination of tears.
Journal Title
Shimane journal of medical science
Volume
22
Issue
2
Start Page
45
End Page
49
ISSN
03865959
ISSN(Online)
24332410
Published Date
2005-12-01
NCID
AA00841586
Publisher Aalternative
Shimane University Faculty of Medicine
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
Remark
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html
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