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language
eng
Author
Akane, Atsushi
Matsubara, Kazuo
Shiono, Hiroshi
Nakamura, Hiroaki
Hasegawa, Masanori
Kagawa, Masato
Takahashi, Setsunori
Description
Southern blot analysis using probes labeled with digoxigenin-11-2'-deoxyuridine-5'-triphosphate (digoxigenin-11-dUTP) instead of ^<32>P-labeled deoxyribonucleotide was investigated. A molecular weight standard marker λ-HindIII should have been labeled with pure digoxigenin-11-dUTP instead of the DIG DNA labeling mixture containing both digoxigenin-11-dUTP and 2'-deoxythymidine-5'-triphosphate. For the labeling of probes, nick translation using pure digoxigenin-11-dUTP was more suitable than random primer extension using the labeling mixture. Furthermore, some modifications on stringency were discussed to identify hybridization signals of the target genes more clearly.
Subject
Southern blot analysis
non-radioactive labeling
digoxigenin-11-2'-deoxyuridine-5'-triphosphate
Journal Title
Shimane journal of medical science
Volume
13
Start Page
1
End Page
9
ISSN
03865959
ISSN(Online)
24332410
Published Date
1992
NCID
AA00841586
Publisher Aalternative
Shimane Medical University
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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