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File
language
eng
Author
Yamasaki, Yukikazu
Ohta, Yukari
Park, Jae Gweon
Matsuda, Hideyuki
Description
Enterobacter sp. G-1 produced both chitinolytic and chitosanolytic enzymes when the carbon source was slightly deacetylated chitin or its oligomer, but not when the carbon source in a medium was highly deacetylated chitosan or its oligomer. Synthesis of these enzymes was not inhibited by chitosan, but was promoted in proportion to the amount of added chitin. The synthesis was induced by insoluble chitin or by its oligomers, but not by its monomer. These results indicate that Enterobacter sp. G-1 produces both chitinolytic and chitosanolytic enzymes in response to an N acetyl group in chitin, and that when there are more N-acetyl groups the production of the enzyme is higher, which led to more cell divisions. The results of cultures in which GlcNAc2 is the only carbon source led us to conclude that GlcNAc2 does not directly enter the cell. Instead, GlcNAc derived from GlcNAc2 is metabolized. However, GlcNAc did not induce production of both enzymes. These results imply that Enterobacter sp. G-1 secretes the enzymes after recognizing insoluble chitin or its oligomers. Then the monomer enters the cell and is metabolized by the bacteria.
Key words: Enterobacter sp. G 1; chitinolytic and chitosanolytic enzymes; chitin; chitosan.
Journal Title
Bulletin of the Faculty of Agriculture, Shimane University
Volume
28
Start Page
107
End Page
117
ISSN
0370940X
Published Date
1994-12-20
NCID
AN00108015
Publisher
島根大学農学部
Publisher Aalternative
Shimane University, Faculty of Agriculture
NII Type
Departmental Bulletin Paper
OAI-PMH Set
Shimane University
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