Histological Detection of mmcp-4 Expressing Cells by In Situ RT-PCR Method

( 最大 2000 件 )
URI http://ir.lib.shimane-u.ac.jp/metadata/38274
ファイル
公開状態
学外公開
内容記述(抄録等)

Mast cells are highly heterogenic cells meaning that different genes are expressed in each different population of mast cells. So far, it has been very difficult to detect that which individual mast cell expresses which genes in situ. In this study, we amplified the mouse mast cell protease (mmcp)-4 mRNAs by in situ RT-PCR method and successfully visualized the signals of amplicons. It was found that the cells expressing mmcp-4 mRNA were connective tissue type mast cells but not mucosal type mast cells, in mouse tongue and intestine sections. This is the first report that the cells expressing the mast cell-specific gene were detected on histological specimens by in situ RT-PCR method. And it is suggested that this method will be a powerful tool for clarifying the relationships between pathological and physiological changes in diseases and many genes expressed in mast cells.

本文言語
英語
タイトル
Histological Detection of mmcp-4 Expressing Cells by In Situ RT-PCR Method
作成者
著者 田中 徹也
チョシャ タナカ テツヤ
Author Tanaka Tetsuya
著者 Asano Satoshi
チョシャ
Author
著者 新林 友美
チョシャ ニイバヤシ トモミ
Author Nibayashi Tomomi
著者 臼田 春樹
チョシャ ウスダ ハルキ
Author Usuda Haruki
著者 和田 孝一郎
チョシャ ワダ コウイチロウ
Author Wada Koichiro
掲載誌名
Shimane journal of medical science
33
1
開始ページ
31
終了ページ
38
並び順
5
ISSN
03865959
発行日
2016-09-30
NCID
AA00841586
SID
9174
主題
in situ RT-RCR
mast cells
mouse mast cell protease
DCMI
text
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
区分
島根大学
掲載誌コード
Shimane journal of medical science
学位授与番号